Age of pigs | Treatment group |
---|
 | A | B | C | D | E | F |
---|
D7 | 0 | 0 | 0 | 0 | 0 | 0 |
D28 | 0A,C (35.5)a | 17A (36.2)a | 89B (54.9)b | 9A (32.7)a | 0C (8.34)c | 0 |
D56 | 100A (76.4)a | 100A (75.5)a | 100A (89.3)b | 100A (73.4)a | 0B (14.1)c | 0 |
D70 | 100A (92.6)a | 100A (92.7)a | 100A (93.5)a | 100A (93.6)a | 60B (63.6)b | 0 |
D84 | 100 (96.4)a | 100 (96.3)a | 100 (96.7)a | 100 (96.4)a | 100 (84.9)b | 0 |
- Legend: Serology was performed using DAKO® M.hyo ELISA (Oxoid Limited, Hampshire, UK) for Mycoplasma hyopneumoniae in each group (Groups A-E: n = 14 pigs per group; group F: n = 8 pigs)
- Group A (F7.2C), B (F20.1L), C (B2V1W20 1A-F), D (J strain), E (positive control; non-vaccinated, challenge infected), F (negative control; non-vaccinated, non-challenged).
- Sera with IP value > 50% was considered positive, IP values < 50% were considered negative. Intermediate IP values were considered doubtful and classified as negative.
- Within a row, different superscript letters correspond to significant differences between the groups (P < 0.05): capital letters for the proportion of seropositive pigs, lowercase letters for the IP values. Group F was not included in the statistical analysis.