Table 1 Percent of positive RT-QuIC reactions and time to threshold (ThT) at different sample dilution levels and under different testing conditions
From: RT-QuIC detection of chronic wasting disease prion in platelet samples of white-tailed deer
Dilution | NaCl | NaI | NaCl-ASR1 | NaI-ASR1 | ||||
|---|---|---|---|---|---|---|---|---|
% | ThT | % | ThT | % | ThT | % | ThT | |
Samples from CWD + deer | ||||||||
10–1 | 0.00 | (N/A) | 4.35 | (56.33 ± 4.65) | 24.64 | (19.75 ± 10.99) | 18.84 | (37.79 ± 18.19) |
10–2 | 57.97 | (29.11 ± 19.40) | 63.77 | (27.64 ± 15.84) | 60.87 | (22.84 ± 16.27) | 82.61 | (21.55 ± 11.84) |
10–3 | 37.68 | (35.10 ± 18.72) | 52.17 | (28.79 ± 16.98) | 34.78 | (26.85 ± 15.16) | 69.57 | (30.19 ± 15.54) |
Samples from CWD- deer | ||||||||
10–1 | 0.00 | (N/A) | 1.11 | (47.00*) | 1.11 | (27.50*) | 0.00 | (N/A) |
10–2 | 1.11 | (69.25*) | 2.22 | (56.00 ± 12.37) | 4.44 | (60.81 ± 7.30) | 8.89 | (52.03 ± 11.16) |
10–3 | 4.44 | (56.44 ± 11.15) | 1.11 | (39.25*) | 2.22 | (46.25 ± 15.91) | 2.22 | (46.00 ± 31.11) |