Comparative biotoxicity study for identifying better alternative insecticide especially green nano-emulsion which used as mosquitocides

Table 1 The sequences of the forward and reverse primer used in the quantitative real-time PCR

Animal Genes	Gene Sequence [5’-3’]	Accession number	Reference
IL-6 (Rats)	F- 5-AGTAGTGAGGAACAAGCCAGAGC	NM_012589	Qing He et al. 2021.
IL-6 (Rats)	R- TTGGGTCAGGGGTGGTTATTG	NM_012589	Qing He et al. 2021.
IL-1β (Rats)	F- GTGGCAATGAGGATGACTT	XM_032902343	Qing He et al. 2021.
IL-1β (Rats)	R- TGGGCTTATCATCTTTCAA	XM_032902343	Qing He et al. 2021.
GAPDH (Rats)	F- ACTTTGGTATCGTGGAAGGACTCAT	NM_001009784	Puech et al. 2015.
GAPDH (Rats)	R-GTTTTTCTAGACGGCAGGTCAGG	NM_001009784	Puech et al. 2015.
TNFα-1. (Fishes)	F-GGTTAGTTGAGAAGAAATCACCTGCA	NM_001279533.1	Praveen et al. 2006.
TNFα-1. (Fishes)	R-GTCGTCGCTATTCCCGCAGATCA	NM_001279533.1	Praveen et al. 2006.
IL-1β (Fishes)	F- TGCACTGTCACTGACAGCCAA	DQ061114.1	Heinecke and Buchmann 2013.
IL-1β (Fishes)	R- ATGTTCAGGTGCACTATGCGG	DQ061114.1	Heinecke and Buchmann 2013.
β-Actin F (Fishes)	F-CAGCAAGCAGGAGTACGATGAG	XM_003455949.2	Akbari et al. 2017.
β-Actin F (Fishes)	R-TGTGTGGTGTGTGGTTGTTTTG	XM_003455949.2	Akbari et al. 2017.

ISSN: 1746-6148