Fig. 2From: Brachyspira in dogs: risk factors of shedding in central Germany and longitudinal study of an infected kennelImmunohistochemistry for “B. canis” (A, B, E, G) and B. pilosicoli (C, D, F, H, I) in canine colon (A-D), canine duodenum (E, F) and porcine colon (G-I). A: Moderate numbers of “B. canis”-immunoreactive bacteria on the luminal surface of the colon. B: Higher magnification from A showing the characteristic spiral-shaped morphology of Brachyspira (arrow). C: Minimal numbers of B. pilosicoli-immunoreactive bacteria on the luminal surface of the colon. D: Higher magnification from B showing the characteristic spiral-shaped morphology of Brachyspira (arrow). E: No detection of “B. canis”-immunoreactive bacteria in the duodenum. F: No detection of B. pilosicoli-immunoreactive bacteria in the duodenum. G: Minimal numbers of “B. canis”-immunoreactive, spiral-shaped bacteria on the luminal surface of the colon of a pig with intestinal spirochetosis associated with the detection of B. pilosicoli by PCR. H: Large numbers of B. pilosicoli-immunoreactive, spiral-shaped bacteria on the luminal surface of the colon of a pig with intestinal spirochetosis associated with the detection of B. pilosicoli by PCR. I: Higher magnification from H showing the characteristic spiral-shaped morphology of Brachyspira (arrow). Immunohistochemistry employing polyclonal rabbit anti-“B. canis”-antibody or rabbit anti-B. pilosicoli-antibody and the avidin-biotin-peroxidase complex method with DAB chromogen and hematoxylin counterstain. Differential interference contrast microscopy. Bars A, C, E, F = 100 μm; B, D, I = 20 μm; G, H = 200 μmBack to article page