Fig. 1

Schematic diagrams of the constructs used in the study. (A) The full-length cDNA clone of CDV ZJ. The full-length viral cDNA was flanked by hammer-head ribozyme (HamRz) and hepatitis delta ribozyme (HdvRz) sequences at both terminals of the viral genome. The six overlapping fragments and the overlapping regions are shown below the genome. Transcription of the plasmid is under the control of the CMV promoter and SV40 polyA signal. (B) Strategy for constructing the CDV minigenome. The minigenome is composed of the 3’ leader, the N gene start signals (GS), the noncoding region (NCR) of the N gene, EGFP, the 5′ NCR of the L gene, the L gene end signals (GE) and the 5′ trailer, which was inserted into the same vector used for the generation of vial cDNA clones. (C) Co-expression of CDV N, P, and L genes in one plasmid. The plasmid pCMV-3MCS was derived from the pUC57 vector by introducing three CMV promoters and polyadenylation signals, which contained three independent expression cassettes for multiple gene expression. Cloning strategies and individual plasmids (pCMV-N, pCMV-P, pCMV-NP, pCMV-L, and pCMV-NPL) are shown