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Table 1 Reproducibility analysis of the real-time RT-RPA assay using different RNA standards

From: Rapid detection of goose astrovirus genotypes 2 using real-time reverse transcription recombinase polymerase amplification

C

(copies/μL)

Intra-assay

CV (%)

Inter-assay

CV (%)

TT value

mean ± SD

TT value

mean ± SD

1 × 105

7.00 ± 0

0

6.92 ± 0.37

5.35

1 × 104

8.24 ± 0.13

1.63

8.44 ± 0.31

3.71

1 × 103

10.72 ± 0.38

3.58

10.92 ± 0.37

3.39

1 × 102

13.58 ± 0.38

2.82

13.72 ± 0.38

2.79

  1. TT threshold time
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BMC Veterinary Research

ISSN: 1746-6148

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