Fig. 3From: Rapid detection of goose astrovirus genotypes 2 using real-time reverse transcription recombinase polymerase amplificationSensitivity analysis of the real-time RT-RPA assay. Serial ten-fold dilutions of the RNA standard ranging from 105 to 100 copies/μL were analyzed using the real-time RT-RPA assay. The results showed that the RNA standard from 105 to 102 copies/μL was determined to be positive between 4 and 14 min and no fluorescent signals were detected from 101 and 100 copies/μL of RNA standard, demonstrating that the detection limit was 102 copies/μLBack to article page