Fig. 6

Palmatine restrains the migration and metastasis of CMT cells. A-C CMT-U27 cells grown in 6-well plates and scratched by 1000 μl tip. Then, CMT-U27 cells cultured in fresh media containing various concentrations of palmatine for 24 h at 37ºC. Images showing CMT-U27 cells migration after palmatine treatment in a dose- and time-dependent manner and quantification of the number of migrated cells over time. Magnification, 40 × . Values are mean ± SD. ** p < 0.01, *** p < 0.001 versus untreated cells by one-way ANOVA followed by Bonferroni post-hoc test. D Fluorescent images showing the lymph nodes of treatment and control groups stained for cytokeratin and LYVE-1. Scale bar, 400 μm. E–G Magnified images and quantification of lymph node metastases in the cortex and medulla regions in the control and treatment groups. The dotted lines demarcate the margin of the lymph node. Scale bar, 50 μm. Values are mean ± SD. * p < 0.05, ** p < 0.01 versus control by one-way ANOVA followed by Bonferroni post-hoc test. Con., control; Pal., palmatine