Table 9 Oligonucleotide primers used in PCR to confirm the diagnosis of beta-hemolytic streptococci
Primers | Nucleotide sequences | Target gene | Size (bp) |
|---|---|---|---|
16S_F 16S_R | 5’-AGAGTTTGATCMTGGCTCAG-3’ 5’-GCTGCCTCCCGTAGGAGT-3’ | 16 S rRNA | 354 |
SeM_F SeM_R | 5’-CAGAAAACTAAGTGCCGGTG-3’ 5’-ATTCGGTAAGAGCTTGACGC-3’ | SeM | 541 |
sodA_F sodA_R | 5’-CAGCATTCCTGCTGACATTCGTCAGG-3’ 5’-CTGACCAGCATTATTCACAACCAGCC-3’ | sodA | 235 |
Kin_F Kin_R | 5’-TCAAATCGGTTGGCACAGAC-3’ 5’-CGTCCTTAGCATAGAAGGATTGG-3’ | Streptokinase | 279 |
SeeI_F SeeI_R | 5’-GAAGGTCCGCCATTTTCAGGTAGTTTG-3’ 5’-GCATACTCTCTCTGTCACCATGTCCTG-3’ | SeeI | 520 |