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Fig. 4 | BMC Veterinary Research

Fig. 4

From: Evaluation of Substance P concentrations in the blood plasma of jugular and tail vein of healthy German Simmental cows

Fig. 4

Study protocol for blood sampling in cows for the trial Blood samples were taken over a period of two days. For the basal substance P concentration (BJV) sampling time was at 7:45 a.m. of the first study day (D1) from the Vena jugularis. For the determination of a circadian rhythm of substance P additional blood samples (TV1—TV5) were taken from the Vena caudalis mediana with intervals of six hours (8:00 a.m., 02:00 p.m., 08:00 p.m. on d1 and 2:00 a.m. and 8:00 a.m. on day 2 (D2) of the study). Clinical examination and fecal sampling were performed after sampling of the BJV and TV1 at 8:15 a.m. on D1

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