Molecular surveillance and genetic diversity of Anaplasma spp. in cattle (Bos taurus) and goat (Capra aegagrus hircus) from Hainan island/province, China

Table 1 Primers used for the amplification of 16S rRNA, MSP4 and Gro EL

Primer	Sequence 5’ to 3’	Assay	Target gene	Amplicon size (bp)	References
EE-1	TCCTGGCTCAGAACGAACGCTGGCGGC	Anaplasma	16S rRNA	1433	Barlough et al., 1996 [54]
EE-2	AGTCACTGACCCAACCTTAAATGGCTG	Anaplasma	16S rRNA	1433	Barlough et al., 1996 [54]
SSAP2f	GCTGAATGTGGGGATAATTTAT	A. phagocytophilum	16S rRNA	641	Kawahara et al., 2006 [55]
SSAP2r	ATGGCTGCTTCCTTTCGGTTA	A. phagocytophilum	16S rRNA	641	Kawahara et al., 2006 [55]
AB1f	CTCGTAGCTTGCTATGAGAAC	A. bovis	16S rRNA	551	Kawahara et al., 2006 [55]
AB1r	TCTCCCGGACTCCAGTCTG	A. bovis	16S rRNA	551	Kawahara et al., 2006 [55]
AC1f	CTGCTTTTAATACTGCAGGACTA	A.centrale	16S rRNA	426	Kawahara et al., 2006 [55]
AC1r	ATGCAGCACCTGTGTGAGGT	A.centrale	16S rRNA	426	Kawahara et al., 2006 [55]
MSP45	GGGAGCTCCTATGAATTACAGAGAATTGTTTAC	A.ovis/A.marginale	MSP4	851	de la Fuente et al., 2006 [56]
MSP43	CCGGATCCTTAGCTGAACAGGAATCTTGC	A.ovis/A.marginale	MSP4	851	de la Fuente et al., 2006 [56]
groELf	TGAAGAGCATCAAACCCGAAG	A. capra	Gro EL	876	Yang et al., 2016 [57]
groELr	CTGCTCGTGATGCTATCGG	A. capra	Gro EL	876	Yang et al., 2016 [57]

ISSN: 1746-6148