Table 1 Summary of immunohistochemical methodology
Primary antibody (Clone) | Type | Dilution | Supplier | Antigen retrieval | Blocking solution | Secondary antibody (dilution) |
|---|---|---|---|---|---|---|
CD68 (EBM11) | Monoclonal anti-human | 1:100 | Dako | Protease from Bacillus licheniformis 0.64% in PBS for 8 min | BSA 1% | Anti-Mouse (1:200) |
iNOS | Polyclonal Rabbit | 1:500 | NeoMarkers, Fremont CA | Citrate pH 6.0 oven | NGS 10% | Anti-Rabbit (1:200) |
HLA-DR antigen Alpha-chain, clone TAL.1B5 | Monoclonal anti-human | 1:20 | Dako | Citrate buffer pH 3.2 in microwave | NGS 10% | Anti-Mouse (1:200) |