Fig. 3From: Monitoring the immune response of macrophages in tuberculous granuloma through the expression of CD68, iNOS and HLA-DR in naturally infected beef cattleExpression of macrophages (CD68+). CD68+ staining in (a): stage I and (b): stage II granulomas in the lymph node of beef cattle naturally infected with Mycobacterium. Heavy positive staining can be observed within the cytoplasm of macrophages (arrows) and multi-nucleated giant cells (arrowheads). (IHC, 50 μm). (c): Scattered CD68+ macrophage cells can be detected within the rim of inflammatory cells surrounding the necrotic center of the stage III granuloma (arrows). (d): Expression of CD68+ macrophages in a stage IV granuloma (arrows). (IHC, 100 μm). The expression of CD68+ macrophages can be seen within the cytoplasm of a few epithelioid, macrophages, and multi-nucleated giant cells (arrow head) surrounding the necrotic center. (IHC, 100 μm)Back to article page