Table 1 Primer sequences used for real-time quantitative PCR
Target gene | Primer sequence (5′-3′) | Product length (bp) | Accession number | |
|---|---|---|---|---|
Forward (Tm) | Reverse (Tm) | |||
Nestin SOX2 GFAP MBP β3-tubulin | TTCCAACCCTTCTCTCGGCT (59.7) AACCCCAAGATGCACAACTC (58.3) CTGCGGCTAGACCAACTCAC (60.7) GCAGATGTGGAGCAGAACAA (58.4) AGCACGGTATAGACCCCAGT (59.0) | CAAGGGTATCAGGCAAGGGC (60.1) CGGGGCCGGTATTTATAATC (55.8) CCAGATCCAGACGGGCTAAG (59.6) GTCCTCTTGGATGGTCTGGA (58.4) TCCGTGTAGTGACCTTTGGC (58.6) | 137 152 187 225 244 | XM_025430810.2 XM_038445642.1 XM_038676213.1 XM_038653864.1 XM_038666722.1 |