Fig. 7

Comparison of gene expression in cells before and after differentiation. Medium 3 was used to differentiate cells. (A) Heat map of mRNA expression (B) Relative mRNA expression of NSPCs and differentiation markers. The data were obtained from experiments biologically repeated two times, with each bar representing an average of the gene expression calculated with the formula 2^−ΔΔCT; CT value was normalized to the housekeeping gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and mRNA expression is shown relative to the expression in the P3 group (passage 3 cells). The error bar represents standard deviation. * denotes significance compared with the P3 group at p < 0.05. ^ denotes significance compared with the P6 group at p < 0.05. # denotes significance compared with the P9 group at p < 0.05