Fig. 6

Immunocytochemistry of differentiated neural stem/progenitor cells (NSPCs). Medium 3 was used to differentiate cells 3rd, 6th, and 9th passage cells. (A) astrocyte marker glial fibrillary acidic protein (GFAP) (red), culture for 14 DIV. (B) immature neuron marker β-3 tubulin (red), culture for 14 DIV. (C) mature oligodendrocyte marker myelin basic protein (MBP) (green), culture for 21 DIV. Nuclei were stained with DAPI (blue). (D) Each well of the 24-well plate was immunostained with single marker and quantified. The quantification was performed by calculating the average percentage of GFAP⁺, β-3 tubulin⁺, and MBP⁺ cells out of the total cells in three wells. (E) Comparison of gene expression of differentiated cells (passage 3). The data were obtained from experiments biologically repeated two times, with each bar representing an average of the gene expression calculated with the formula 2^−ΔΔCT; CT value was normalized to the housekeeping gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and mRNA expression is shown relative to the expression in the GFAP group. The error bar represents standard deviation. * denotes significance compared with the GFAP group at p < 0.05. ^ denotes significance compared with the MBP group at p < 0.05