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Fig. 5 | BMC Veterinary Research

Fig. 5

From: Neural stem/progenitor cells from adult canine cervical spinal cord have the potential to differentiate into neural lineage cells

Fig. 5

Immunocytochemistry of neural stem/progenitor cells (NSPCs) cultured as neurospheres or monolayers. (A) 3rd, 6th, and 9th passage cells cultured in a monolayer for 5 DIV. (B) 3rd, 6th, and 9th passage cells cultured as neurosphere for 5 DIV. Nuclei were stained with DAPI (blue) and NSPCs marker for SOX2 (green) and nestin (red). (C) Each well of the 24-well plate was immunostained with nestin and SOX2. The quantification was performed by calculating the average percentage of nestin+/SOX2+ cells out of the total cells in three wells. Quantification was carried out with cells cultured in monolayer

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BMC Veterinary Research

ISSN: 1746-6148

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