Fig. 1

Neurospheres obtained by suspension culture of (A) cells at passage 3, (B) cells at passage 6, and (C) cells at passage 9. The scale bar represents 200 μm. (D) Scheme of experiment: neural stem/progenitor cells (NSPCs) were serially passaged in monolayer from passage 0 to 10. Cells that were cultured with monolayer were additionally cultured in neurospheres form only in 3rd, 6th, and 9th passage cells for microscopic and immunostaining observation. Growth curve and cell differentiation analysis were conducted in monolayer