Table 1 Gene-specific primer sequences
From: Immunomodulation by cannabidiol in bovine primary ruminal epithelial cells
Gene ID | Gene Name | Sequence (5’-3’)1 | Amplicon Size (bp) | Efficiency (%)2 | Target RefSeq3 |
|---|---|---|---|---|---|
Housekeeping | Â | Â | Â | Â | Â |
GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | F: GGGTCATCATCTCTGCACCT R: GGAGGCATTGCTGACAATCT | 101 | 91 | NM_001034034.2 |
STX5 | Syntaxin 5 | F: CCATTCAGAGGATCGACGAG R: GGATGTGACCGACTGGAAGT | 95 | 103 | NM_001075444.1 |
Targets | Â | Â | Â | Â | Â |
CASP4 | Caspase 4 | F: CACTCGTCTGGCTCTCATCA R: GTCCCTGGCTGTGAGTTTCT | 148 | 97 | NM_176638.5 |
CXCL8 | C-X-C motif chemokine ligand 8 | F: AGAGCTGAGAAGCAAGATCCA R: ACCCTACACCAGACCCACAC | 150 | 104 | NM_173925.2 |
CXCL9 | C-X-C motif chemokine ligand 9 | F: ATTTGCTCCAAGCCCTTCTT R: CTTTTGGTTGACCTGTTTCTCC | 136 | 111 | NM_001113172.1 |
CXCL10 | C-X-C motif chemokine ligand 10 | F: CGAGATTATTGCCACAATGAAA R: CTCTTTCCGTGTTCGAGGAG | 130 | 105 | NM_001046551.2 |
IL1B | Interleukin 1 beta | F: CTGAGGAGCATCCTTTCATTC R: GTCCTGGAGTTTGCACTTTAT | 114 | 97 | NM_174093.1 |
IL6 | Interleukin 6 | F: AGTGTGAAAGCAGCAAGGAGA R: AGCAAATCGCCTGATTGAAC | 105 | 112 | NM_173923.2 |
LTA4H | Leukotriene A4 hydrolase | F: GTCAGTGCCAGGCTATCCAC R: TCTTTGGGGACAGACACCTC | 97 | 92 | NM_001034280.1 |
PTGES3 | Prostaglandin E synthase 3 | F: TTGAGGAAAGCGAGAAGAGG R: AAGCAGGTTGCATCGTGAA | 146 | 93 | NM_001007806.2 |
PTGS2 | Prostaglandin-endoperoxidase synthase-2 | F: GGTGTGAAAGGGAGGAAAGA R: GGCAAAGAATGCAAACATCA | 117 | 93 | NM_174445.2 |
TNF | Tumor necrosis factor alpha | F: TGTAGCCGACATCAACTCTCC R: CCCTGAAGAGGACCTGTGAG | 149 | 103 | NM_173966.3 |