Fig. 4

Experiment 1 gene expression of pro-inflammatory cytokines TNF (A), IL1β (B), IL6 (C) and CXCL8 (D), inflammasome regulator CASP4 (E), chemokines CXCL9 (F) and CXCL10 (G), and prostaglandin synthase PTGS2 (H) in primary ruminal epithelial cells in various exposure combinations of cannabidiol (CBD) and lipopolysaccharide (LPS). E1-A through E1-F indicate treatment groups. The ΔCt was used for statistical analysis and results are presented as fold change with exposure treatments held relative to CON within animal. Different letters indicate significance (P < 0.05)