Skip to main content
Fig. 5 | BMC Veterinary Research

Fig. 5

From: Preparation of Escherichia coli ghost of anchoring bovine Pasteurella multocida OmpH and its immunoprotective effect

Fig. 5

Results of ci857-E gene and recombinant protein. A: PCR amplification results of ci857-E gene. M: DL5000 DNA Marker; 1: ci857-E gene; 2: Negative control. B: Identification results of recombinant plasmid E’-OmpH-pET28a-ci857-E. M: DL10,000 DNA Marker; 1: pET28a-Ci857-E digested by BamHI. C: Western blot analysis of recombinant protein E’-OmpH. M: Prestained protein marker; 1: Negative Control; 2: Anti-His antibody as the first antibody. D: SDS-PAGE results of recombinant fusion protein expression. M: Marker; 1 is the E. Coli BL21 (E’-Omph -pET28a-ci857-E) before induce; 2 is first induced E. Coli BL21(E’-Omph -pET28a-ci857-E) by IPTG for 12 h in 28 ℃ then induced by shifting temperature at 42 ℃; 3, 4, 5 is the induced E. Coli BL-21 (E’-Omph -pET28a-ci857-E) by IPTG for 5 h in 37 ℃, 12 h in 28 ℃, 5 h in 28 ℃; 6, 7, 8 is the induced E. Coli Rossta (E’-Omph -pET28a-ci857-E) by IPTG for 5 h in 37 ℃, 12 h in 28 ℃, 5 h in 28 ℃

Back to article page

BMC Veterinary Research

ISSN: 1746-6148

Contact us