Fig. 4

E’-Omph fusion expression identification results. A: PCR amplification results of E’ gene. M: DL5000 DNA marker; 1: Gene E’; 2: Negative Control. B: PCR amplification results of gene OmpH. M: DL2000 DNA Marker; 1: Negative Control; 2: OmpH gene. C: PCR amplification results of gene E’-Omph. M: DL5000 DNA Marker; 1–2: PCR Amplification of E’-Omph combined gene. D: PCR Identification Results of Recombinant Plasmid E’-Omph-pET28a. M: DL10,000 DNA Marker; 1: Negative Control; 2: PCR product of E’-OmpH-pET28a. E: Identification results of recombinant plasmid E’-Omph-pET28a. M: DL10,000 DNA Marker; 1: E’-Omph-pET28a digested by BamH I and XhoI; 2: Negative Control