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Table 5 Effect of four successive weeks supplementation of Lactulose (Vetelact) on Blood Biochemical Components (mean ± SE) in layer chickens

From: Use of lactulose as a prebiotic in laying hens: its effect on growth, egg production, egg quality, blood biochemistry, digestive enzymes, gene expression and intestinal morphology

Variable

Treatment groups

P value

Group 1 (control)

Group 2 (vetelact 0.05 ml/kg)

Group 3 (vetelact 0.1 ml/kg)

Group 4 (vetelact 0.15 ml/kg)

Metabolites. mmol/L

 Glucose

12.22 ± 0.48

12.42 ± 0.27

12.07 ± 0.17

12.35 ± 0.22

0.386

 Creatinine

< 9.00 ± 0.00

< 9.00 ± 0.00

< 9.00 ± 0.00

< 9.00 ± 0.00

 

 Urea

< 0.60 ± 0.00

< 0.60 ± 0.00

< 0.60 ± 0.00

< 0.60 ± 0.00

 

 Cholesterol

2.59 ± 0.10 ab

2.67 ± 0.27 ab

2.87 ± 0.20 a

2.22 ± 0.06 b

0.040

 TBIL

15.00 ± 0.50 a

11.33 ± 0.73 b

11.33 ± 1.36 b

12.00 ± 0.58 b

0.014

Proteins. g/L

 TP

62.00 ± 1.32a

57.67 ± 1.42b

56.67 ± 0.60bc

54.33 ± 0.60c

0.001

 Albumin

20.33 ± 0.33

19.67 ± 0.17

19.67 ± 0.44

19.33 ± 0.44

0.281

 Globulin

42.00 ± 1.32a

38.00 ± 1.26b

37.00 ± 0.29b

35.33 ± 0.17b

0.001

 ALB/GLOB

0.47 ± 0.02b

0.53 ± 0.02a

0.50 ± 0.00ab

0.53 ± 0.02a

0.007

Minerals. mmol/L

 Phosphorus

2.08 ± 0.14b

2.08 ± 0.15b

2.98 ± 0.08a

2.61 ± 0.25a

0.001

 Calcium

> 4.00 ± 0.00

> 4.00 ± 0.00

> 4.00 ± 0.00

> 4.00 ± 0.00

 

Enzymes. U/L

 ALT

< 10.00 ± 0.00

< 10.00 ± 0.00

< 10.00 ± 0.00

< 10.00 ± 0.00

 

 ALKP

114.33 ± 14.86b

186.00 ± 30.09a

224.67 ± 5.53a

199.67 ± 20.42a

0.003

 GGT

35.00 ± 2.75ab

33.00 ± 1.61ab

30.33 ± 2.17b

37.67 ± 2.19a

0.140

 Amylase

211.67 ± 12.72

233.67 ± 14.82

237.67 ± 8.84

213.67 ± 7.50

0.258

 Lipase

69.67 ± 4.44c

107.00 ± 2.65a

86.33 ± 1.67b

90.67 ± 9.59b

0.001

  1. Means within a row superscripted by different letters are significantly different (p < 0.05)
  2. TP total protein, TBIL Total bilirubin, ALB Albumin, GLOB Globulin, ALT alanine transaminase, ALKP Alkaline phosphatase, GGT gamma-glutamyl transferase