Identification and evaluation in-vitro of conserved peptides with high affinity to MHC-I as potential protective epitopes for Newcastle disease virus vaccines

Table 1 Evaluation of the cellular immune response to the 5 selected peptides by ELISpot assay

Antigen in the well of ELISpot plate	Peptide sequence (length)	Name of NDV protein	Spot Counting (Mean ± SD)		Statistical tests 95%
			Control Group	Vaccinated group:	Non-parametric method	Parametric methods
			Spleen cells of non-immunized chickens	Spleen cells of immunized chickens	Kruskal–Wallis	Poisson regression	Negative binomial regression
Peptide 01	FEELIHVNH (9 mers)	L	8.67 ± 5.92	133.67 ± 100.09	0.0065^a	0.000^a	0.000^a
Peptide 02	FEDWVANY (8 mers)	HN	65.33 ± 114.81	209.83 ± 97.78	0.0250^a	0.000^a	0.044^a
Peptide 03	KESVAATNE (9 mers)	F	9.33 ± 10.13	159.5 ± 141.86	0.0039^a	0.000^a	0.000^a
Peptide 04	NEEREAKN (8 mers)	HN	42.33 ± 72.10	90.83 ± 83.23	0.1282	0.000^a	0.241
Peptide 05	KEMIHVNH (8 mers)	L	8.17 ± 3.43	147.5 ± 114.61	0.0039^a	0.000^a	0.000^a
Pool of peptides	Mix of 5 peptides^b	L + HN + F	9.33 ± 5.79	191.67 ± 157.12	0.0039^a	0.000^a	0.000^a
ConA	Positive control		667.33 ± 32.15	798.5 ± 117.17	0.0547	0.000^a	0.002^a
Cells with culture medium	Negative control		3.17 ± 4.02	40.33 ± 39.36	0.0202^a	0.000^a	0.000^a

^aSignificant difference between vaccinated and control groups, ^bMix of 5 peptides in same concentration
NDV Newcastle Disease Virus, ELISpot Enzyme-linked immunospot, ConA Concavalin A, L Large polymerase, HN Hemagglutinin-neuraminidase, F Fusion

ISSN: 1746-6148