Fig. 1From: Characterization of receptor tyrosine kinase activation and biological activity of toceranib phosphate in canine urothelial carcinoma cell linesReceptor tyrosine kinase transcript expression in primary canine urothelial carcinoma tumor samples and primary urothelial carcinoma cell lines. Total RNA was isolated from primary canine urothelial carcinoma (UC) tumors (N = 9) and quantitative RT-PCR was performed to determine the relative expression of A PDGFRα, C PDGFRβ, E KIT, and G VEGFR2 transcript. All reactions were performed in triplicate. Data presented represent the mean, error bars = SD. Total RNA was isolated from canine urothelial carcinoma (UC) cell lines (N = 5) and quantitative RT-PCR was performed to determine the relative expression of B PDGFRα, D PDGFRβ, F KIT, and H VEGFR2 transcript. Three independent experiments were performed for each cell line and all reactions were performed in triplicate. Data presented represent the mean, error bars = SD from three experimental replicatesBack to article page