Recombinant pseudorabies virus with gI/gE deletion generated by overlapping polymerase chain reaction and homologous recombination technology induces protection against the PRV variant PRV-GD2013

Table 3 Specific primer of homologous recombination arm used in prime Script 2step PCR

Primer	Sequence (5′-3′)
P₁	5′- CGGGCTGCAGGAATTCGCGGCCGCCGAGTCGTGGCAGCTGACGCTGA −3′
P₂	5′- ACGTCATCACGAAGGAGCCCAGGCAGCCGCGGAAGGCTTCGT −3′
P₃	5′- ACGAAGCCTTCCGCGGCTGCCTGGGCTCCTTCGTGATGACGT − 3′
P₄	5′- AGGTCGACGGTATCGATATCGATACTCGGTGAGCACCTTCCACA − 3′
P₁′	5′- CGGGCTGCAGGAATTCGCGGCCGCCGAGTCGTGGCAGCTGACGCTGA − 3′
P₂′	5′- CAGGGGATAACGCAGGAAAGAACATAGGCAGCCGCGGAAGGCTTCGT − 3’
P₃′	5′- CAGCTCATTTTTTAACCAATAGGCCGGGCTCCTTCGTGATGACGT − 3’
P₄′	5′- AGGTCGACGGTATCGATATCGATACTCGGTGAGCACCTTCCACA − 3’
P₅′	5′- ACGAAGCCTTCCGCGGCTGCCTATGTTCTTTCCTGCGTTATCCCCTG − 3’
P₆′	5′- ACGTCATCACGAAGGAGCCCGGCCTATTGGTTAAAAAATGAGCTG − 3’
gI-F	5′- GTCCGTAGCCTCCGCAGTACC − 3’
gI-R	5′- CTTCTGGCGCAGCTCGGCCAA − 3’
gE-F	5′- GTGACCACGGTGTGCTTC − 3’
gE-R	5′- ACAGCACGCAGAGCCAGA − 3’
gIF	5′- GTCCGTAGCCTCCGCAGTACC − 3’
gER	5′- AATGCGGGCGGACCGGTTC − 3’

ISSN: 1746-6148