Table 2 Molecular identification and genotyping of Brucella isolates
Method(s) | Reference | Result |
|---|---|---|
PCR AMOS | Bricker &Halling, 1993 | Isolates 6, 7, 11: Brucella melitensis |
Bruce ladder | Lopez-Goni et al. 2008 | Isolate 6: Brucella melitensis Isolate 7: Brucella abortus Isolate 11:Brucella melitensis |
PCR omp31 | Vizcaino et al., 1997 | Isolate 6:positive Isolate 7: negative Isolate 11: positive |
Suis ladder | Lopez-Goni et al., 2011 | Unique pattern for all B. melitensis strains |
MLVA 16 (Bruce06–08 - 11 - 12 - 42 - 43- 45 - 55, 18–19 - 21, 04–07 - 09 - 16 - 30) | La Fleche et al., 2006; Al Dahouk et al., 2007 | Isolate 6: 1–5–3-13-3-2-3-2-4-41-8-5-4-3-7-6 Isolate 7: genotype (1–5–3-13-2-2-3-2-4-41-8-5-4-3-7-6), consistent with B. melitensis |
MLST 9 (gap - aroA - glk - dnaK - gyrB - trpE - cobQ - int-hyp (orf1)-omp25) | Whatmore et al., 2007 | Isolates 6 and 7: sequence type 8, 3–2–3-2-1-5-3-8-2; consistent with B. melitensis |
WGS/wgSNP | Illumina NexteraXT guide (no. 150319425031942), following protocol revision C | DDBJ/ENA/GenBank under accession numbers CVI_6 ChI CP058599, CVI_6 ChII CP058600, CVI_7 ChI CP058597, and CVi_7 ChII CP058598 |