Table 1 List of primers and amplified genes
Primer name | sequences | Gene | Purpose | Amplicon | Reference | Reference strain |
|---|---|---|---|---|---|---|
M30F2/08F | ATGAGYCTTYTAACCGAGGTCGAAACG | M | Detection of AIV type A | 244 | [34] | 26–52 |
M264R3/08R | TGGACAAANCGTCTACGCTGCAG | M | Detection of AIV type A | [34] | 267 − 245 | |
HA5-1 F | AAAGTGATCAGATYTGCATTG | HA | Sequencing | 420 | [20] | 44–64 |
HA5-1R | TGGTATGGRCATGCTGAGCTCA | HA | Sequencing | [20] | 440–461 | |
H5-2 F | TCATTTTGAGAAGATTCTGATCATCC | HA | Sequencing | 754 | This study | 375–400 |
H5-2R | CCCCTGCTCATTGCTATGGT | HA | Sequencing | This study | 1128 − 1109 | |
H5-3 F | GGCAACGTGGAAGAATGGAC | HA | Sequencing | 744 | This study | 617–636 |
H5-3R | ACTCGAAACAACCGTTACCC | HA | Sequencing | [34] | 1360 − 1341 | |
H5-4 F | CATCCACCCTCTCACCATCG | HA | Sequencing |  | [20] | 927–968 |
H5-4R | GCGATCCATTGGAGCACATC | HA | Sequencing |  | [20] | 1681 − 1662 |
NA8-1 F | AATAATGACCGTTGGCTCCA | NA | Detection & sequencing | 616 | This study | 18–37 |
NA8-1R | AGTAGGCACCCCTCCGTAAT | NA | This study | 633 − 614 | ||
NA8-2 F | AAGTGGATGGCGATTGGTGT | NA | Sequencing | 403 | This study | 567–586 |
NA8-2R | TGGGCAACCCTGCACATAAA | NA | Sequencing | This study | 969 − 950 |