Fig. 3

S100A4, αSMA, and vimentin expression in invasive areas in an IMPC of the canine mammary gland. Superresolution images of the nuclear envelope markers Lamin B1/B2 (green), S100A4, αSMA, and Vimentin (red); nuclei were stained with Hoechst (blue) in invasive (a-c) and in situ areas (b). Note that intermediate microfilament staining surrounds the cystic space of micropapillary tumoral areas. The merged images show the overlapping staining. Representative images from n = 4 discrete primary tumor cases are shown, and ten images were acquired from invasive areas in each sample. Scale bars = 20 μm