Real Time 2x-PCR Mix SYBR A | 10 μl |
Starter 1055F 10 μM 5′-ATGGCTGTCGTCAGCT-3’ | 0.4 μl |
Starter 1392R 10 μM 5′-ACGGGCGGTGTGTAC-3’ | 0.4 μl |
DNA template | 1 μl |
deionized water | 8.2 μl |
initial denaturation | 95 °C, 3 min. |
denaturation | 95 °C, 15 s |
priming | 58 °C, 30 s |
elongation | 72 °C, 30s |
appointment of the melting curve (measurement of the fluorescence in each temperature) | 65 °C - > 95 °C |