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Table 1 Optimal serum dilution and HRP-conjugated antibody dilution reacted with 1 ng/100 μl of coating buffer

From: Evaluation of an in-house indirect enzyme-linked immunosorbent assay of feline panleukopenia VP2 subunit antigen in comparison to hemagglutination inhibition assay to monitor tiger antibody levels by Bayesian approach

HRP-Conjugated antibody

Serum dilution / HRP dilution

S/N ratio

1:100

1:200

1:300

1:400

1:500

Anti-cat

1:1000

2.460

3.095a

3.508

3.679

3.951

 

1:2000

2.310

2.724

2.921

3.085

3.122

 

1:3000

2.350

2.641

2.959

2.949

2.983

 

1:4000

2.300

2.730

2.749

2.733

2.826

Anti-tiger

1:1000

3.812

2.736

2.651

2.235

1.949

1:2000

3.160a

2.600

2.134

1.825

1.732

1:3000

2.577

2.132

1.968

1.649

1.603

1:4000

2.651

2.283

2.000

1.582

1.637

  1. a Represents suitable S/N ratio obtained from this study
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BMC Veterinary Research

ISSN: 1746-6148

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