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Table 1 Optimal serum dilution and HRP-conjugated antibody dilution reacted with 1 ng/100 μl of coating buffer

From: Evaluation of an in-house indirect enzyme-linked immunosorbent assay of feline panleukopenia VP2 subunit antigen in comparison to hemagglutination inhibition assay to monitor tiger antibody levels by Bayesian approach

HRP-Conjugated antibody Serum dilution / HRP dilution S/N ratio
1:100 1:200 1:300 1:400 1:500
Anti-cat 1:1000 2.460 3.095a 3.508 3.679 3.951
  1:2000 2.310 2.724 2.921 3.085 3.122
  1:3000 2.350 2.641 2.959 2.949 2.983
  1:4000 2.300 2.730 2.749 2.733 2.826
Anti-tiger 1:1000 3.812 2.736 2.651 2.235 1.949
1:2000 3.160a 2.600 2.134 1.825 1.732
1:3000 2.577 2.132 1.968 1.649 1.603
1:4000 2.651 2.283 2.000 1.582 1.637
  1. a Represents suitable S/N ratio obtained from this study
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