Fig. 4

Effect of the fixative and click reaction on antibody fluorescence and T cell surface antigens. Mononuclear cells isolated from spleen of 1 chicken were divided into 5 tubes that received different treatments. (a) Dot plots representing flow cytometry analysis. (b) Dot plots showing the effect of the fixative and the click reaction on the percentage of CD4⁺ or CD8⁺ cells detected by flow cytometry. (c) Quantitative data showing the effect of the fixative on the percentage of CD4⁺ or CD8⁺ cells detected by flow cytometry. The results are expressed as the mean ± standard deviation of 3 independent experiments. (d) Quantitative data showing the effect of the click reaction on the percentage of CD4⁺ or CD8⁺ cells detected by flow cytometry. The results are expressed as the mean ± standard deviation of 4 independent experiments. All values shown are percentage of singlet cells. Significant differences are indicated by * (p = 0.0286), ns p > 0.05. Per sample, 30,000 events were acquired using a FACSMelody flow cytometer