Number of DNA copies (copies/μL) | triplex real-time PCR Ct Value (mean ± SD) | Uniplex real-time PCR Ct Value (mean ± SD) | ||||
---|---|---|---|---|---|---|
AIV | NDV | DTMUV | AIV | NDV | DTMUV | |
1 × 107 | 13.61 ± 0.20 | 14.50 ± 0.14 | 14.30 ± 0.33 | 13.93 ± 0.07 | 15.01 ± 0.17 | 14.68 ± 0.41 |
1 × 106 | 16.42 ± 0.21 | 18.00 ± 0.37 | 17.87 ± 0.09 | 16.31 ± 0.15 | 18.22 ± 0.26 | 18.05 ± 0.04 |
1 × 105 | 20.33 ± 0.22 | 21.39 ± 0.08 | 21.44 ± 0.09 | 20.32 ± 0.04 | 22.26 ± 0.11 | 21.24 ± 0.16 |
1 × 104 | 23.02 ± 0.52 | 24.41 ± 0.16 | 24.99 ± 0.05 | 23.04 ± 0.44 | 24.71 ± 0.16 | 24.73 ± 0.43 |
1 × 103 | 26.01 ± 0.37 | 28.04 ± 0.35 | 28.30 ± 0.08 | 25.84 ± 0.20 | 27.74 ± 0.53 | 27.78 ± 0.01 |
1 × 102 | 29.55 ± 0.15 | 30.45 ± 0.36 | 31.19 ± 0.65 | 29.30 ± 0.30 | 30.78 ± 0.47 | 31.12 ± 0.12 |
1 × 101 | 32.42 ± 0.68 | 33.34 ± 0.71 | 33.29 ± 0.86 | 32.98 ± 0.34 | 33.85 ± 0.68 | 33.38 ± 0.60 |