Fig. 5From: Development and application of a triplex real-time PCR assay for simultaneous detection of avian influenza virus, Newcastle disease virus, and duck Tembusu virusStandard curves of the triplex real-time PCR assay. The linear range and detection limit of each virus were evaluated by detecting 10-fold serial dilution of the plasmid (from 1 × 107 copies/μL to 1 × 101 copies/μL) against the Ct. The coefficient of determination (R2) and the equation of the regression curve (y) were calculatedBack to article page