Fig. 4

PDPN and LAMA4 transcription and mRNA expression in HSA and normal splenic samples. a Genome browser views (UCSC browser) of the ChRO-seq dataset showing PDPN and LAMA4 gene loci. ChRO-seq reads on sense and anti-sense strands are shown in red and blue, respectively. Red lines indicate the encoding gene in the Ensemble database. b RT-PCR analysis documenting PDPN and LAMA4 transcript expression in normal (1: B001NM, 2: B004NM, 3: B006NM) and HSA samples corresponding to ChRO-seq data (C085, C340 and C356). GAPDH primers were used as a positive control. Negative control (NC) lacks cDNA template. Results show that PDPN and LAMA4 mRNA do not appear to be expressed in normal spleen. LAMA4 transcripts were observed in all three HSA samples while PDPN showed strong expression in one of the three HSA samples