Dietary treatment1 (n = 60) | Group (n = 30) | Days of age | |||||
---|---|---|---|---|---|---|---|
10 | 28 | 42 (experimental infection) | 47 | 49 | 56 | ||
DLML | 0-DLML | Vaccination against ND | Vaccination against ORT | 02 | Collection of samples of blood and organs from 7 turkeys per group for immunological (CD4+, CD8+, CD4+CD8+, CD4+CD25+, CD4+IL-6+,IgM+) and biochemical analyses (IgA, IL-6) | Vaccination against ORT | Collection of blood samples from 23 turkeys per group for serological analyses (antibody against ORT and NDV) |
HE-DLML | + 3 | ||||||
MHAL | 0-MHAL | 0 | |||||
HE-MHAL | + | ||||||
DLMH | 0-DLMH | 0 | |||||
HE-DLMH | + | ||||||
MHAH | 0-MHAH | 0 | |||||
HE-MHAH | + |