Fig. 2

Western blotting analysis of the specificity of purified rApd. a Purified rApd was used as antigen and primary antibodies were the anti-histidine monoclonal antibody and H. parasuis-positive pig serum. Lane 1, Purified rApd; Lane 2, Purified fraction from a transformant including vector pET-25b. b Purified rApd was used as antigen, and primary antibodies were antisera of 15 serovar H. parasuis reference strains, isolate CF7066, and four other porcine pathogenic organisms. c The rApd antiserum was used as primary antibody, and antigens were bacterial proteins of 15-serotype H. parasuis reference strains and isolate CF7066. d The rApd antiserum was used as primary antibody, and antigens were five non-typeable H. parasuis isolates and seven other porcine pathogenic organisms. MW, molecular weight. H. parasuis S1-S15, H. parasuis reference strains of serotype 1–15. Asterisks and triangles indicate negative and positive results, respectively