Fig. 1
From: Characterization of five newly derived canine osteosarcoma cell lines
Alkaline phosphatase staining of Osteosarcoma Cell Lines. Alkaline phosphatase (ALP) staining. All cell lines were stained using a commercially available alkaline phosphatase kit. All cell lines, including the previously immortalized canine OS cell lines, Abrams and BWKOS, as well as the normal osteoblast cell line, TNO, stained positive for ALP in 100% of the cells. Variable staining was seen in each cell line with BWKOS and TOM having the lightest staining and Abrams and TOK having the darkest staining, similar to that seen in TNO