Fig. 1From: Novel polymerase spiral reaction assay for the visible molecular detection of porcine circovirus type 3Sensitivity of the polymerase spiral reaction (PSR) and loop mediated isothermal amplification for the detection of Porcine circovirus type 3 (PCV3). Ten-fold serial dilutions of standard plasmid (PCV3-ORF2) were subjected to the PSR and LAMP assays and analyzed. Lane M, molecular size marker DL2000. Lanes 1–7, dilutions of PCV3-ORF2 (1.13 × 107 copies, 1.13 × 106 copies, 1.13 × 105 copies, 1.13 × 104 copies, 1.13 × 103 copies, 1.13 × 102 copies, and 1.13 × 101 copies); and lane N, negative control. a Agarose gel electrophoresis demonstrating the sensitivity of the PSR assay. b Colorimetric analysis demonstrating the sensitivity of the PSR assay. c Agarose gel electrophoresis demonstrating the sensitivity of the LAMP assayBack to article page