Table 4 Instrumental conditions used for validating the analytical method and subsequently used in the screening of adrenal gland
Analyte (correspondent IS in brackets) | tR (min) | Precursor Ion | DP (V) | Target | Qualifier | ||||
|---|---|---|---|---|---|---|---|---|---|
Fragment | CE (V) | Fragment | (qualifier/quantifier %) | CE (V) | |||||
6β-hydroxyprednisolone (A) | 4.78 | 377.1 | 47 | 341.1 | 11 | 323.2 | 28% | 15 | |
20α-dihydroprednisolone (A) | 11.28 | 363.3 | 37 | 345.2 | 15 | 309.3 | 75% | 18 | |
20β-dihydroprednisolone (A) | 12.58 | 363.3 | 37 | 345.2 | 15 | 309.3 | 24% | 18 | |
20β-dihydroprednisone (A) | 12.87 | 361.3 | 55 | 153.0 | 21 | 313.2 | 54% | 16 | |
Prednisolone (A) | 17.16 | 361.3 | 55 | 343.3 | 16 | 325.3 | 38% | 16 | |
Prednisone (A) | 17.68 | 359.1 | 70 | 341.1 | 17 | 313.2 | 41% | 19 | |
Cortisol (B) | 17.72 | 363.2 | 69 | 121.3 | 33 | 147.3 | 67% | 44 | |
Cortisone (C) | 18.64 | 361.2 | 83 | 163.4 | 36 | 105.3 | 14% | 64 | |
Internal standards | |||||||||
A | Prednisolone D6 | 16.95 | 367.1 | 48 | 330.3 | 16 | – | – | – |
B | Cortisol D2 | 17.68 | 365.2 | 65 | 121.8 | 39 | – | – | – |
C | Cortisone D2 | 18.61 | 363.1 | 67 | 165.1 | 34 | – | – | – |