Fig. 2

Dot blot assay prepared using antibodies raised against E. coli-expressed BEFV G1 region. a Recombinant G1 protein expression was induced for 0, 2, and 4 h in E. coli and analyzed by SDS-PAGE (upper panel). Rabbit anti-BEFV antiserum was employed to identify the 42 kDa rG1 protein (lower panel). b Protein samples of BEFV culture supernatant (BEFV sup.), rG1 protein (rG1), and BHK-21 cell culture supernatant (BHK-21 sup.) were separated by SDS-PAGE (upper panel) and probed by rabbit antiserum raised against the E. coli-expressed BEFV G1 region (lower panel). c Dot blot assays were set up with raised rabbit anti-rG1 or anti-BEFV antiserum as primary antibodies. Two-fold serial dilutions of BEFV test samples, or BHK-21 cell supernatant, were applied for BEFV detection