Fig. 1

Growth characteristics of CDV-3 and CDV-11 in Vero and DH82 cells. Vero cells were infected with CDV-3 (c) and CDV-11 (e) at a MOI of 0.1, DH82 cells were infected with CDV-3 (d) and CDV-11 (f) at a MOI of 0.1, Vero (a) and DH82 cells (b) with no CDV infection were served as controls. Cells were incubated for 3 days and then fixed and stained with CDV monoclonal antibody specific for nucleoprotein. (Magnification, × 200) (g) Growth curves of CDV were performed in Vero and DH82 cells. Cells were infected with CDV-3 or CDV-11 at a MOI of 0.1 and incubated at 37 °C. Viruses were collected at 0, 24, 48, 72, and 96 h p.i. and viral titers tested as described in Materials and Methods. Experiments were both carried out in triplicate