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Table 5 primer and probe sequence design for the GPS RPA and RPA-LF assays

From: Rapid and simple detection of Glaesserella parasuis in synovial fluid by recombinase polymerase amplification and lateral flow strip

Assay

Name

Sequence

Amplicon size

RPA

infBRPAF

CATTGAATCAGCWGGYCCATCAATTCCTGTGGA

307 bp

infBRPAR

CACTTTTACTTCTGCCGTTGAAAGCTCGTGTAAA

RPA-LF

infBRPARB

(B)CACTTTTACTTCTGCCGTTGAAAGCTCGTGTAAA

infBRPAFB

(F)ACTTTCAGGCGTACCAGCCGCAGGTGATGAAGC(H)GACAGTGGTGCGTGAT(3)

  1. Features: B = Biotin label; F = 6-carboxyfluorescein (FAM) label; H = abasic tetrahydrofuran (THF) residue; 3 = C3 spacer. All primers were designed according to the instructions from TwistDx (http://www.twistdx.co.uk)
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BMC Veterinary Research

ISSN: 1746-6148

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