Fig. 6From: An evaluation of TAZ and YAP crosstalk with TGFβ signalling in canine osteosarcoma suggests involvement of hippo signalling in disease progressionEffect of TAZ or YAP knockdown plus and minus TGFβ on cell viability of canine OSA cell lines. Graphs depict the fluorescence readings at 530/590 nm when TAZ (a) or YAP (b) was depleted in OVC-cOSA-75, OVC-cOSA-31, and D17 cells, which were stimulated or not with 5 ng/mL TGFβ in the presence (+ Doxo) or absence (−Doxo) of Doxorubicin. Readings were first blank-corrected to fluorescence values obtained for the media only control. Bars depict the average ± SEM from two independent experiments plated in duplicate. Asterisks depict statistical differences as determined by a one-way ANOVA (Kruskal-Wallis test) with Dunn-Sidak correction to identify significant differences relative to control within each, the –Doxo and + Doxo treatment groups; * indicates p < 0.05, ** indicates p < 0.010. TAZ knockdown decreased cell viability in the D17 cell line both in the presence and absence of Doxorubicin. YAP knockdown decreased cell viability in the presence (OVC-cOSA-75, OVC-cOSA-31) and absence (all three cell lines) of DoxorubicinBack to article page