Fig. 5From: An evaluation of TAZ and YAP crosstalk with TGFβ signalling in canine osteosarcoma suggests involvement of hippo signalling in disease progressionAll canine OSA cell lines express TGFβRI, TGFβRII, respond to TGFβ1 treatment and have basal levels of Hippo mediators TAZ and YAP. Cells were serum starved for 6 h and either stimulated with 5 ng/mL TGFβ1 (+) or not (−) for 24 h. Representative immunoblots and densitometry analysis showing the levels of TGFβRI and TGFβRII (a), pSmad3 and Smad 3 (b), pSmad2 and Smad2 (c) and TAZ and YAP (d), n = 3. Phospho-protein levels were normalized to native protein levels, and all native protein levels were normalized to β actin (loading control) and compared to the control (−) to determine relative differences. Independent t-test was used to determine significant differences between control and treated groups, * indicates p < 0.05, ** indicates p < 0.010, error bars depict average ± SEMBack to article page