Table 1 Oligonucleotide primers used for screening bat samples of Coxiella burnetii, Bartonella spp., Rickettsia spp., Ehrlichia spp. and Anaplasma spp.
From: Coxiella and Bartonella spp. in bats (Chiroptera) captured in the Brazilian Atlantic Forest biome
Pathogen | Target gene | Oligonucleotide primer | Primer sequence (5′ – 3′) | Amplicon size (bp) | Cycling conditions | Reference |
|---|---|---|---|---|---|---|
Coxiella burnetii | IS1111 | Outer primer F Outer primer R Nested primer F Nested primer R | TATGTATCCACCGTAGCCAGC CCCAACAACACCTCCTTATTC AAGCGTGTGGAGGAGCGAACC CTCGTAATCACCAATCGCTTCGTC | 687 bp 440 bp | 95 °C for 5 min, 40 cycles of 95 °C for 30s, 60 °C for 30s, 72 °C for 1 min, final extension of 72 °C for 7 min 95 °C for 5 min, 30 cycles of 95 °C for 30 s, 66 °C for 30 s, 72 °C for 30 s, final extension of 72 °C for 5 min | [36] |
Bartonella spp. | gltA | Outer primer F Outer primer R | GCTATGTCTGCVTTCTATCAYGA AGAACAGTAAACATTTCN GTHGG | 731 bp | 95 °C for 10 min, 35 cycles of 95 °C for 30s, 58 °C for 30s, 72 °C for 1 min, final extension of 72 °C for 8 min | [35] |
Rickettsia spp. | gltA | Outer primer F Outer primer R Nested primer F Nested primer R | CATCCTATGGCTATTATGCTTGC TATACTCTCTATG(T/A)AC(A/G)T(A/G)ACC CTTACCGCTATTAGAATGATTGC GAGCGA(T/G)AGCTTCAAG(T/C)TCTAT | 885 bp 572 bp | 95 °C for 10 min, 30 cycles of 95 °C for 30 s, 55 °C for 40 s, 72 °C for 55 s, final extension of 72 °C for 10 min 95 °C for 7 min, 25 cycles of 95 °C for 30 s, 63 °C for 30 s, 72 °C for 35 s, final extension of 72 °C for 10 min | [35] [35] |
Ehrlichia spp. /Anaplasma spp. | 16 s rRNA | Outer primer F Outer primer R | GGTACCYACAGAAGAAGTCC TGCACTCATCGTTTACAG | 345 bp | 95 °C for 3 min, 35 cycles of 95 °C for 15 s, 55 °C for 30 s, 72 °C for 30s, final extension of 72 °C for 5 min | [38] |