Table 1 Sequences of primers and probes for FMDV RT-PCR, real-time RT-PCR and LFS RT-RPA assays
Assay | Primers and probes | Sequence 5´-3´ | Amplicon size (bp) | References |
|---|---|---|---|---|
RT-PCR | 3D-F | CCCATTGAGTATCTACGAGG | 1104 | This study |
| Â | 3D-R | CAACGCAGGTAAAGTGATC | Â | Â |
real-time | FMDV-F | ACTGGGTTTTACAAACCTGTGA | 86 | [7] |
RT-PCR | FMDV-R | GCGAGTCCTGCCACGGA | Â | Â |
| Â | FMDV-P | FAM-TCCTTTGCACGCCGTGGGAC-BHQ1 | Â | Â |
LFS | FMDV-LFS-F | TTGGTCACTCCATTACCGATGTCACTTTCCTC | 258 | This study |
RT-RPA | FMDV-LFS-R | 5’-Biotin-AACGCAGGTAAAGTGATCTGTAGCTTGGAAT |  |  |
|  | FMDV-LFS-P | 5’-FAM-GCACGCCGTGGGACCATACAGGAGAAGTT |  |  |
|  |  | GAT(THF)TCCGTGGCAGGACTCG-C3-spacer-3’ |  |  |