Fig. 1

Schematic illustration of the protein microarrays for the detection of antibodies against IBV. Step 1, the prepared chip was rinsed thrice with PBST; Step 2, 100 μL of diluted serum was added and incubated on a constant temperature oscillator and then washed with PBST thrice; Step 3, 100 μL of goat anti-chicken IgY conjugated to HRP was added, and the plate was incubated on a constant temperature oscillator and washed with PBST thrice; Step 4, for chemiluminescence, 15 μL of chemiluminescent substrate was added to each well, and images were taken at a wavelength of 645 nm with Amersham Imager 600; Step 5, for RDT, 60 μL of TMB was added to each well and incubated for 5 min in a dark place; then, the results were observed