Table 3 List of primary antibodies used for immunohistochemistry and western blotting analysis
From: Expression of vascular endothelial growth factor (VEGF) in equine sarcoid
Antibody | Manufacturer | Clone | Host species | Antigen retriever | IHC Dilution | WB Dilution |
|---|---|---|---|---|---|---|
vWF | DAKO | Polyclonal | Rabbit | 0.05% pepsin in 0.01 M HCl (pH 2) at 37 ° C for 15 min | 1:400 | 1:1000 |
bcl-2 | DAKO | 124 | mouse | Heated Citrate buffer, ph 6.0, 30 min | 1:100 | 1:1000 |
VEGF | Thermoscientific | JH121 | mouse | Heated Citrate buffer, ph 6.0, 30 min, | 1:200 | 1:200 |
Ki67 | Santa Cruz | D-6 | mouse | Heated Citrate buffer, ph 6.0, 30 min, | 1:100 | 1: 1000 |