Table 1 Bacterial strains and plasmids used in this study
Genotype / characteristics | |
|---|---|
E. coli strains | |
TG1 | E. coli cloning host |
pMD18-T-HCE-MCS-rrnBT1T2/TG1 | TG1harbring pMD18-T-HCE-MCS-rrnBT1T2,Cmrr |
pGBHC-Pupp/TG1 | TG1 harboring pGBHC-Pupp, Cmrr |
pUC57-LFcin/JM109 | JM109 harboring pUC57-Lfcin, Amr (Synthesis by Genewiz Biological Technology Co., Ltd) |
pGBHCupp-TF-LFEC-TR/TG1 | TG1 harboring pGBHCupp-TF-ELFEC-TR, Cmrr |
L. caseistrains | |
ΔuppL.caseiATCC393 | L. casei ATCC393 derivative without upp |
pGBHCupp-TF-LFEC-TR/L.casei | ΔuppL.caseiATCC393 harboring pGBHCupp-TF-ELFEC-TR, Cmrr |
ΔthyAL.casei-LFEC | ΔthyAL.casei ATCC393 with LfcinBexpression cassette insertion in the thyA |
Plasmids | |
pGBHC-upp | Ori (pWV01) with a copy of upp expression cassette, Cmrr |
pGBHCupp-TF-LFEC-TR | pGBHC-upp containing LfcinB expression cassette between upstream and downstream sequence flanking the thyA integration site |